Investigating immunosensor for determination of SD-biomarker-Aβ based on AuNPs/AC@PANI@CS modified electrodes with amplifying the signal.

Bolu Sun, Lei Kan, Chengyang Gao, Hongxia Shi, Lin Yang, Tiankun Zhao, Quhuan Ma, Xiaofeng Shi, Chunyan Sang

Journal: Analytical biochemistry 2022;661():114996

PMID: 36427556

Full Text on Elsevier Science View on PubMed

Abstract

Sleep debt (SD) is one of the important triggers for causing not only physiological and mental illness but also dangerous work. Therefore, achieving an early and objective assessment of SD is of great significance in the precaution against SD-related diseases and unsafe work. Here, an ultrasensitive electrochemical immunosensor was constructed for analysis of SD biomarker amyloid-β (Aβ). The gold nanoparticles/chitosan-coated polyaniline-functionalized activated carbon (AuNPs/AC@PANI@CS) composites were employed as the sensing platforms. Since PANI and AC can form an effective conductive path, it can effectively enhance the penetration of electrolytes on the electrode surface and the rapid transport of charges and ions, significantly enhancing the electrochemical response signal of the immunosensor. Under the optimized experimental conditions, the fabricated immunosensor had a wide linear range of 1.95 pg mL to 1000.00 pg mL, with a low detection limit of 0.014 pg mL. This study not only provides an effective method for the accurate and rapid detection of Aβ, but also offers a novel evaluation strategy for the objective assessment of SD and the study of related pathological mechanisms.

Address: School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou, 730050, China. Electronic address: [email protected].; School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou, 730050, China.; Lanzhou Zhongjianke Testing Technology Co., Ltd, Lanzhou, 730000, Gansu, China.; School of Life Science and Engineering, Wenzhou Engineering Institute of Pump&Valve, Lanzhou University of Technology, Lanzhou, 730050, China. Electronic address: [email protected].; Gansu Academy of Medical Science, Lanzhou, 730050, China.; Gansu Academy of Medical Science, Lanzhou, 730050, China. Electronic address: [email protected].; CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, 730000, China.

DOI: 10.1016/j.ab.2022.114996

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MeSH Terms: Humans, Sleep Deprivation, Gold, Biosensing Techniques, Immunoassay, Metal Nanoparticles