Sensitive biosensors based on topological insulator BiSe and peptide.

Yujiu Jiang, Peng Zhu, Jinge Zhao, Shanshan Li, Yetong Wu, Xiaolu Xiong, Xu Zhang, Yuxiang Liu, Jiangyue Bai, Zihang Wang, Shiqi Xu, Minxuan Wang, Tinglu Song, Zhiwei Wang, Weizhi Wang, Junfeng Han

Journal: Analytica chimica acta 2023;1239():340655

PMID: 36628700

Full Text on Elsevier Science View on PubMed

Abstract

In this work, we designed a facile and label-free electrochemical biosensor based on intrinsic topological insulator (TI) BiSe and peptide for the detection of immune checkpoint molecules. With topological protection, BiSe could have robust surface states with low electronic noise, which was beneficial for the stable and sensitive electron transport between electrode and electrolyte interface. The peptides are easily synthesized and chemically modified, and have good biocompatibility and bioavailability, which is a suitable candidate as the recognition units for immune checkpoint molecules. Therefore, the peptide/BiSe was developed as a suitable working electrode for the electrochemical biosensor. The basic performance of the designed peptide/BiSe biosensor was investigated to determine the Anti-HA Tag Antibody and PD-L1 molecules. The linear detection range was from 3.6 × 10 mg mL to 3.6 × 10 mg mL, and the detection limit was 1.07 × 10 mg mL. Moreover, the biosensor also displayed good selectivity and stability.

Address: Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China.; Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Key Laboratory of Cluster Science of Ministry of Education, Beijing Key Laboratory of Photoelectronic/Electro-photonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081, China.; Department of Rheumatology, China-Japan Friendship Hospital, Beijing, 100029, China.; Experimental Centre of Advanced Materials School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, China.; Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China. Electronic address: [email protected].; Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing, 100081, China; Yangtze Delta Region Academy of Beijing Institute of Technology, Jiaxing, 314019, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing, 100081, China. Electronic address: [email protected].

DOI: 10.1016/j.aca.2022.340655

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MeSH Terms: Biological Availability, Electrodes, Electron Transport, Immune Checkpoint Proteins, Peptides, Biosensing Techniques