A "Turn-On" fluorescent probe for detection and removal of Zn in aqueous and its application in living cells.

Huizhen Wang, Tao Yang, Shaofei Ni, Zhengfeng Xie, Guanjun Chang

Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2022;280():121501

PMID: 35749973

Full Text on Elsevier Science View on PubMed

Abstract

Using 3-hydroxy-2-naphthoic acid hydrazine and 4-(diethylamino) salicylaldehyde. as raw materials, compound L with an acylhydrazones structure was synthesized. The structure of compound L was characterized by nuclear magnetic resonance spectroscopy, X-ray single crystal diffraction, Fourier transform infrared spectroscopy, and mass spectrometry. The results show that Compound L can quickly and selectively recognize zinc ions in the HO/DMSO (V:V = 3:7) solvent system. After that, the spectral performance of probe L was studied by fluorescence spectroscopy and UV-vis spectroscopy. The results show that the combination with Zn can significantly enhance the fluorescence intensity of probe L while being almost unaffected by other coexisting ions. After that, Job's curve method, nuclear magnetic titration analysis, and mass spectrometry were used to study the binding mechanism of probe L and Zn. The results showed that probe L coordinated with Zn is 1:1. The linear equations of different concentrations of Zn and fluorescence intensity were obtained by fitting, and the detection limit of probe L for Zn was determined to be 6.75 × 10 mol/L. The experimental study of standard addition and recovery showed that probe L could be used for the quantitative detection of Zn in natural water samples. After that, we prepared L-doped sodium alginate hydrogel (SAL). The research results show that SAL has obvious adsorption capacity for Zn in solution, and the color change before and after adsorption can be easily distinguished by the naked eye under ultraviolet light. SEM-EDS study showed that the microscopic morphology and composition of SAL changed significantly before and after adsorption. This fluorescent probe can be used for detection and removal of Zn in aqueous solution. Also, probe L is effective for sensing for zinc (II) in living tumor cells. Overall, this work allows us to obtain a great potential to be applied to detect and remove Zn.

Address: State Key Laboratory of Environment-friendly Energy Materials, National Engineering Technology Center for Insulation Materials, School of Material and Chemistry, Southwest University of Science and Technology, Mianyang, 621010, P. R. China; School of Science, Xihua University, Chengdu 610039, China. Electronic address: [email protected].; Laboratory of Human Diseases and Immunotherapy, and State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Institute of Immunology and Inflammation, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.; Department of Chemistry and Key Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province, Shantou University, Shantou, Guangdong 515063, China. Electronic address: [email protected].; School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, 610500, China.; State Key Laboratory of Environment-friendly Energy Materials, National Engineering Technology Center for Insulation Materials, School of Material and Chemistry, Southwest University of Science and Technology, Mianyang, 621010, P. R. China. Electronic address: [email protected].

DOI: 10.1016/j.saa.2022.121501

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S1386...

MeSH Terms: Fluorescent Dyes, Ions, Spectrometry, Fluorescence, Water, Zinc