Developing Ganoderma lucidum as a next-generation cell factory for food and nutraceuticals.

Fidelis Azi, Zhe Wang, Wenhao Chen, Dewei Lin, Peng Xu

Journal: Trends in biotechnology 2024;42(2):197-211

PMID: 37659953

Full Text on Elsevier Science View on PubMed

Abstract

Ganoderma lucidum holds a colossal reservoir of hydrolytic enzymes and therapeutic compounds and can be a sustainable source of proteins and bioactive compounds. Its metabolic versatility, propelled by its rich genome content, provides excellent biosynthetic machinery for innovation-driven pathway engineering. However, robust regulatory networks and low frequency of homologous recombination are critical bottlenecks that limit the development of molecular tools and precise genetic markers for biomanufacturing innovations in this organism. Modern synthetic biology provides tools that could help to accelerate precise multiple gene targeting and editing and untangling the biosynthetic machinery of G. lucidum. This review provides insight into molecular strategies to unwind the regulatory bottlenecks and transform G. lucidum into efficient cell factories for food and nutraceuticals.

Address: Department of Chemical Engineering, Guangdong Technion-Israel Institute of Technology (GTIIT), Shantou, Guangdong 515063, China; Technion-Israel Institute of Technology, Haifa 3200002, Israel.; Institute of Agro-Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Department of Chemical Engineering, Guangdong Technion-Israel Institute of Technology (GTIIT), Shantou, Guangdong 515063, China; Technion-Israel Institute of Technology, Haifa 3200002, Israel; Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion, GTIIT, Shantou, Guangdong 515063, China. Electronic address: [email protected].

DOI: 10.1016/j.tibtech.2023.07.008

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MeSH Terms: Reishi, Dietary Supplements