Screening for inhibitors of transcription factors using luciferase reporter gene expression in transfected cells.
Deborah Phippard, Anthony M Manning
Journal: Methods in molecular biology (Clifton, N.J.)
2003;225():19-23
PMID: 12769470
Address:
Roche Pharmaceuticals, Palo Alto, CA, USA.
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MeSH Terms:
DEAE-Dextran ,
Gene Expression Regulation, Neoplastic ,
Genes, Reporter ,
Humans ,
Immunosuppressive Agents ,
Indicators and Reagents ,
Insect Proteins ,
Jurkat Cells ,
Luciferases ,
Organic Chemicals ,
Phytohemagglutinins ,
Recombinant Fusion Proteins ,
Tetradecanoylphorbol Acetate ,
Transcription Factors ,
Transfection
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