Impact of vitamin D supplementation on modulating heat-shock proteins in postmenopausal women: a randomized, double-blind, placebo-controlled study.

Claudio Lera Orsatti, Fábio Lera Orsatti, João Pedro Esteves Alves de Souza, Eliana Aguiar Petri Nahas

Journal: Menopause (New York, N.Y.) 2023;30(7):758-765

PMID: 37220771

Abstract

OBJECTIVE

The aim of this study was to examine the effect of vitamin D (VitD) supplementation on serum heat-shock proteins (HSP) in postmenopausal women (PW).

METHODS

In this double-blind, placebo-controlled trial, 160 PW aged 45 to 65 years with amenorrhea 12 months or more were randomized into two groups: 80 PW in VitD group (oral supplementation with 1,000 IU VitD 3 /d) or 80 PW in placebo group. The PW were assessed at baseline and after 10 months of intervention. Plasma concentrations of 25-hydroxyVitD (25[OH]D) were measured by high-performance liquid chromatography. HSP27/pS78/pS82, HSP27/total, HSP60, HSP70/72, and HSP90α levels were assessed in serum using a multiplexed bead immunoassay.

RESULTS

HSP27 (pS78/pS82 [ P < 0.035] and total [ P < 0.001]) levels increased in the supplemented group when compared with the control group. There was no effect of VitD supplementation on HSP60, HSP70/72, and HSP90α levels.

CONCLUSIONS

Vitamin D supplementation increases serum HSP27 level in PW.

Address: Applied Physiology, Nutrition and Exercise Research Group (PhyNEr), Institute of Health Sciences, Federal University of Triangulo Mineiro (UFTM), Uberaba, Brazil.; From the Department Health Science, Oeste Paulista University-UNOESTE, Jaú, Brazil.; Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University-UNESP, São Paulo, Brazil.

DOI: 10.1097/GME.0000000000002197

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MeSH Terms: Humans, Female, Vitamin D, Postmenopause, HSP27 Heat-Shock Proteins, Vitamin D Deficiency, Cholecalciferol, Dietary Supplements, Double-Blind Method, Heat-Shock Proteins