The synergistic effect of IBRC-M10790 and vitamin D3 on -induced inflammation.

BACKGROUND

Owing to the emergence and spread of multidrug resistance mechanisms in , achieving a successful eradication has become exceedingly difficult. Thus, this study for the first time determines the effect of a combination of vitamin D3 and probiotic on the pathogenesis and treatment of .

METHODS

We established an experimental system using AGS human gastric carcinoma cells and explored the synergistic effect of IBRC-M10790 and vitamin D3 on . Live and pasteurized , -derived membrane vesicles (MVs), and cell-free supernatant (CFS), as well as their combination with vitamin D3 were used during this study. We assessed the anti-inflammatory and anti-oxidative effects of these combinations using RT-qPCR and ELISA, respectively. We further performed an adhesion assay to evaluate the influence of and vitamin D3 on the adherence rate of to AGS cells.

RESULTS

Our results demonstrated that and vitamin D3 possess anti-inflammatory and anti-oxidative effects against infection in AGS cells. The combination of vitamin D3 with the probiotic strain (particularly live and its CFS) can more efficiently reduce the expression of pro-inflammatory cytokines IL-6, IL-8, IFN-γ, and TNF-α in the AGS cells. Moreover, vitamin D3 and exhibited an additive impact preserving the integrity of the epithelial barrier by increasing the expression of the tight junction protein ZO-1. Furthermore, this combination can potentially reduce adherence to AGS cells.

CONCLUSIONS

This study indicates the advantage of combining vitamin D3 and probiotic to attenuate -induced inflammation and oxidative stress. Consequently, probiotic and vitamin D3 co-supplementation can be considered as a novel therapeutic approach to manage and prevent infection.

Copyright © 2023 Nabavi-Rad, Jamshidizadeh, Azizi, Yadegar, Robinson, Monaghan and Zali.