Enhanced folate binding of cultured fibroblasts from Alzheimer's disease patients.

Emanuela Cazzaniga, Alessandra Bulbarelli, Elena Lonati, Francesca Re, Gloria Galimberti, Elena Gatti, Marina Pitto, Carlo Ferrarese, Massimo Masserini

Journal: Neuroscience letters 2008;436(3):317-20

PMID: 18406523

Full Text on Elsevier Science View on PubMed

Abstract

We compared the levels of serum folate from Alzheimer's disease (AD) patients and from age-matched healthy subjects and used primary cultures of fibroblasts, obtained from the two groups, to assess possible differences in their ability to bind folate. The results show that the levels of circulating folate are significantly (p<0.01; n=30) lower in AD patients than in controls (4.91+/-2.44 and 7.56+/-2.5 ng/mL, respectively). Moreover, the folate binding of AD fibroblasts is significantly (p<0.01; n=8) higher (2-4-fold) with respect to controls. RT-PCR experiments suggest that the higher folate binding could be due to an enhanced expression in AD fibroblasts of folate receptor alpha.

Address: Department of Experimental Medicine, University of Milano-Bicocca - Via Cadore 48, 20052 Monza, MI, Italy. [email protected]

DOI: 10.1016/j.neulet.2008.03.046

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MeSH Terms: Aged, Aged, 80 and over, Alzheimer Disease, Carrier Proteins, Cells, Cultured, Female, Fibroblasts, Folate Receptors, GPI-Anchored, Folic Acid, Gene Expression Regulation, Humans, Male, Mental Status Schedule, RNA, Messenger, Radioactivity, Receptors, Cell Surface