ALB, HP, OAF and RBP4 as novel protein biomarkers for identifying cured patients with pulmonary tuberculosis by DIA.

Qiqi Lu, Jun Liu, Yi Yu, Hong-Feng Liang, Shan-Qiang Zhang, Zhi-Bin Li, Jia-Xi Chen, Qiu-Gui Xu, Ji-Cheng Li

Journal: Clinica chimica acta; international journal of clinical chemistry 2023;535():82-91

PMID: 35964702

Full Text on Elsevier Science View on PubMed

Abstract

BACKGROUND

Pulmonary tuberculosis (TB) is a serious infectious disease that lacks robust blood-based biomarkers to identify cured TB. Some discharged patients are not fully cured and may relapse or even develop multidrug-resistant TB. This study is committed to finding proteomic-based plasma biomarkers to support establishing laboratory standards for clinical TB cure.

METHODS

Data-independent acquisition (DIA) was used to obtain the plasma protein expression profiles of TB patients at different treatment stages compared with healthy controls. Multivariate statistical methods and bioinformatics were used to analyze the data.

RESULTS

Bioinformatic analysis suggests coagulation dysfunction and vitamin and lipid metabolism disturbances in TB. Albumin (ALB), haptoglobin (HP), out at first protein homolog (OAF), and retinol-binding protein 4 (RBP4) can be used to establish a diagnostic model for the efficacy evaluation of TB with an area under the curve of 0.963, which could effectively distinguish untreated TB patients from cured patients.

CONCLUSIONS

Our research demonstrated that ALB, HP, OAF and RBP4 can be potential biomarkers for evaluating the efficacy of TB. These findings may provide experimental data for establishing the laboratory indicators of clinical TB cure and providing clinicians with new targets for exploring the underlying mechanisms of TB pathogenesis.

Address: The Medical Research Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan 512025, China.; The Medical Research Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan 512025, China; The Central Laboratory, Yangjiang People's Hospital, Yangjiang 529500, China.; The Central Laboratory, Yangjiang People's Hospital, Yangjiang 529500, China.; The Medical Research Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan 512025, China; The Central Laboratory, Yangjiang People's Hospital, Yangjiang 529500, China; Institute of Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China.; Taizhou Hospital of Zhejiang Province affiliated to Wenzhou Medical University, Taizhou 318050, China; Institute of Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China.; The Medical Research Center, Yue Bei People's Hospital, Shantou University Medical College, Shaoguan 512025, China; The Central Laboratory, Yangjiang People's Hospital, Yangjiang 529500, China; Institute of Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, China. Electronic address: [email protected].

DOI: 10.1016/j.cca.2022.08.002