Two new umbravirus-like associated RNAs (ulaRNAs) discovered in maize and johnsongrass from Ecuador.

Diego F Quito-Avila, Edison G Reyes-Proaño, Alma Mendoza, Paolo Margaria, Wulf Menzel, Sayanta Bera, Anne E Simon

Journal: Archives of virology 2022;167(10):2093-2098

PMID: 35821148

Abstract

Two new umbravirus-like associated RNAs (ulaRNAs) were found, respectively, in maize and Johnsongrass samples from Ecuador. The complete sequences consist of 3,053 and 3,025 nucleotides, respectively, and contain four open reading frames (ORFs). Their genome sequences were 58% identical to each other and 28 to 60% identical to the most closely related viruses. Phylogenetic analysis using full genome sequences and amino acid sequence of the RNA-dependent-RNA polymerase (RdRp) placed both sequences in a clade sharing the most recent common ancestor with ulaRNAs from sugarcane and maize, suggesting that they belong to a monophyletic grass-infecting lineage. Their terminal regions exhibit features common to umbraviruses and ulaRNAs.

Address: Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador. [email protected].; Centro de Investigaciones Biotecnológicas del Ecuador, CIBE, Escuela Superior Politécnica del Litoral, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador. [email protected].; Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador.; INIAP, Estación Experimental Portoviejo, Km 12, Via a Santa Ana, Manabí, Ecuador.; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7 B, Braunschweig, Germany.; Department of Cell Biology and Molecular Genetics, University of Maryland College Park, College Park, MD, 20742, USA.

DOI: 10.1007/s00705-022-05525-4

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https://dx.doi.org/10.1007/s00705-022-05525-4

MeSH Terms: Ecuador, Genome, Viral, Open Reading Frames, Phylogeny, RNA, RNA, Viral, Sorghum, Tombusviridae, Zea mays