The relevance of urolithins-based metabotyping for assessing the effects of a polyphenol-rich dietary intervention on intestinal permeability: A post-hoc analysis of the MaPLE trial.

Tomás Meroño, Gregorio Peron, Giorgio Gargari, Raúl González-Domínguez, Antonio Miñarro, Esteban Vegas-Lozano, Nicole Hidalgo-Liberona, Cristian Del Bo', Stefano Bernardi, Paul Antony Kroon, Barbara Carrieri, Antonio Cherubini, Patrizia Riso, Simone Guglielmetti, Cristina Andrés-Lacueva

Journal: Food research international (Ottawa, Ont.) 2022;159():111632

PMID: 35940768

Free Full Text on Archivio Istituzionale della Ricerca Unimi - Access Free Full Text View on PubMed

Abstract

A polyphenol-rich diet reduced intestinal permeability (IP) in older adults. Our aim was to evaluate if participants categorized according to urolithin metabotypes (UMs) exhibited different responses in the MaPLE trial. Fifty-one older adults (mean age: 78 years) completed an 8-week randomized-controlled-crossover trial comparing the effects of a polyphenol-rich vs. a control diet on IP, assessed through zonulin levels. Plasma and urinary metabolomics were evaluated with a semi-targeted UHPLC-MS/MS method. Gut microbiota was characterized by 16S rRNA gene profiling. UMs were determined according to urolithin excretion in 24 h urine samples. Multivariate statistics were used to characterize the differences in metabolomic and metataxonomic responses across UMs. Thirty-three participants were classified as urolithin metabotype A (UMA), 13 as urolithin metabotype B (UMB), and 5 as urolithin metabotype 0 (UM0) according to their urinary excretion of urolithins. Clinical, dietary, and biochemical characteristics at baseline were similar between UMs (all p > 0.05). After the polyphenol-rich diet, UMB vs. UMA participants showed a 2-fold higher improvement of zonulin levels (p for interaction = 0.033). Moreover, UMB vs. UMA participants were characterized for alterations in fatty acid metabolism, kynurenine pathway of tryptophan catabolism, and microbial metabolization of phenolic acids. These changes were correlated with the reduction of zonulin levels and modifications of gut microbes (increased Clostridiales, including, R. lactaris, and G. formicilis). In conclusion, urolithin-based metabotyping identified older adults with a higher improvement of IP after a polyphenol-rich diet. Our results reinforce the concept that UMs may contribute to tailor personalized nutrition interventions.

Address: Biomarkers and Nutrimetabolomics Laboratory, Department de Nutrició, Ciències de l'Alimentació i Gastronomia, Food Innovation Network (XIA), Institut de Recerca en Nutrició i Seguretat Alimentària (INSA), Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona (UB), 08028 Barcelona, Spain; Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, Madrid 28029, Spain.; Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.; Genetics, Microbiology and Statistics Department, University of Barcelona, 08028 Barcelona, Spain.; Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, United Kingdom.; Geriatria, Accettazione Geriatrica e Centro di Ricerca per l'Invecchiamento, IRCCS INRCA, 60127 Ancona, Italy.; Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy. Electronic address: [email protected].; Biomarkers and Nutrimetabolomics Laboratory, Department de Nutrició, Ciències de l'Alimentació i Gastronomia, Food Innovation Network (XIA), Institut de Recerca en Nutrició i Seguretat Alimentària (INSA), Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona (UB), 08028 Barcelona, Spain; Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, Madrid 28029, Spain. Electronic address: [email protected].

DOI: 10.1016/j.foodres.2022.111632

Link outs

Free resources

Full Text Sources:

MeSH Terms: Acer, Aged, Humans, Hydrolyzable Tannins, Permeability, Polyphenols, RNA, Ribosomal, 16S, Tandem Mass Spectrometry