Association between serum 5-methyltetrahydrofolate and homocysteine in Chinese hypertensive participants with different MTHFR C677T polymorphisms: a cross-sectional study.

Yu Cheng, Shuai Liu, Duo Chen, Yiman Yang, Qiongyue Liang, Ya Huo, Ziyi Zhou, Nan Zhang, Zhuo Wang, Lishun Liu, Yun Song, Xiangyi Liu, Yong Duan, Xiuwen Liang, Bingjie Hou, Binyan Wang, Genfu Tang, Xianhui Qin, Fangrong Yan

Journal: Nutrition journal 2022;21(1):29

PMID: 35562805

Free Full Text on BioMed Central View on PubMed

Abstract

BACKGROUND AND AIMS

Clarifying the association between 5-methyltetrahydrofolate and homocysteine and the effect pattern of methylene tetrahydrofolate reductase (MTHFR C677T) may contribute to the management of homocysteine and may serve as a significant reference for a randomized controlled trial of 5-methyltetrahydrofolate intervention. This study aimed to reveal the association between these two biochemical indices.

METHODS

Study population was drawn from the baseline data of the China Stroke Primary Prevention Trial (CSPPT), including 2328 hypertensive participants. 5-methyltetrahydrofolate and homocysteine were determined by stable-isotope dilution liquid chromatography-tandem mass spectrometry and automatic clinical analyzers, respectively. MTHFR C677T polymorphisms were detected using TaqMan assay. Multiple linear regression was performed to evaluate the association between serum 5-methyltetrahydrofolate and homocysteine.

RESULTS

There was a significant inverse association between 5-methyltetrahydrofolate and homocysteine when 5-methyltetrahydrofolate was ≤ 10 ng/mL, and this association was modified by MTHFR C677T (per 1-ng/mL increment; All: β = - 0.50, P < 0.001; CC: β = - 0.14, P = 0.087; CT: β = - 0.20, P = 0.011; TT: β = - 1.19, P < 0.001). Moreover, the decline in trend in genotype TT participants was stronger than in genotype CC participants (P for difference < 0.001) and genotype CT participants (P for difference < 0.001), while there was no significant difference between genotype CC and genotype CT participants (P for difference = 0.757).

CONCLUSIONS

Our data showed a non-linear association between serum homocysteine and 5-methyltetrahydrofolate among Chinese hypertensive adults, however, it could be inversely linearly fitted when serum 5-methyltetrahydrofolate was ≤ 10 ng/mL, and this association was modified by MTHFR C677T.

Address: State Key Laboratory of Natural Medicines, Research Center of Biostatistics and Computational Pharmacy, China Pharmaceutical University, Nanjing, 210000, China.; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.; Department of Cardiology, Peking University First Hospital, Beijing, China.; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.; Institute of Biomedicine, Anhui Medical University, Hefei, China.; BeiJing TongRen Hospital, CMU, Beijing, China.; Yunnan Key Laboratory of Laboratory Medicine, Kunming, China.; Department of Clinical Laboratory, the First Affiliated Hospital of Kunming Medical University, Kunming, China.; China Mongolia Hospital of Hulunbuir, Inner Mongolia, China.; The Fourth Central Hospital of Baoding City, Hebei, Baoding, China.; Institute of Biomedicine, Anhui Medical University, Hefei, China.; Shenzhen Evergreen Medical Institute, Shenzhen, China.; School of Health Administration, Anhui Medical University, Hefei, China.; Division of Nephrology, Nanfang Hospital, Southern Medical University, National Clinical Research Center for Kidney Disease; State Key Laboratory of Organ Failure Research; Guangdong Provincial Institute of Nephrology, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, 510000, China. [email protected].; State Key Laboratory of Natural Medicines, Research Center of Biostatistics and Computational Pharmacy, China Pharmaceutical University, Nanjing, 210000, China. [email protected].

DOI: 10.1186/s12937-022-00786-w

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MeSH Terms: Adult, Cross-Sectional Studies, Genotype, Homocysteine, Humans, Hypertension, Methylenetetrahydrofolate Reductase (NADPH2), Tetrahydrofolates