The thnI gene is not required for thurincin H biosynthesis or immunity.

Julio Armando Massange-Sánchez, Mario Alberto Núñez-Valle, José E Barboza-Corona, Luz Edith Casados-Vazquez

Journal: Archives of microbiology 2022;204(6):344

PMID: 35596088

Abstract

Thurincin H is a bacteriocin produced by Bacillus thuringiensis, it is encoded in a group of ten genes, most of which have been characterized experimentally or by homology. However, the activity of the thnI gene encoding a 95 amino acid ORF remains unknown. In this work, the thnI gene was cloned under the regulation of two promoters and transformed into a sensitive strain to determine if it acts as an immunity protein. In addition, a deletion mutant without the thnI gene was used to test whether thnI is required or not for the biosynthesis of thurincin H. It was concluded that thnI does not provide immunity and is not required to produce thurincin H.

Address: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Unidad de Biotecnología Vegetal, 44270, Guadalajara, Mexico.; Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico.; Food Department, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico.; Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico. [email protected].; Food Department, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico. [email protected].; Cátedra Conacyt, Universidad de Guanajuato, Guanajuato, Mexico. [email protected].

DOI: 10.1007/s00203-022-02938-2

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https://dx.doi.org/10.1007/s00203-022-02938-2

MeSH Terms: Bacillus thuringiensis, Bacterial Proteins, Bacteriocins, Promoter Regions, Genetic