Accelerated biocorrosion of stainless steel in marine water via extracellular electron transfer encoding gene phzH of Pseudomonas aeruginosa.

Enze Zhou, Mingxing Zhang, Ye Huang, Huabing Li, Jianjun Wang, Guangming Jiang, Chengying Jiang, Dake Xu, Qiang Wang, Fuhui Wang

Journal: Water research 2022;220():118634

PMID: 35691192

Full Text on Elsevier Science View on PubMed

Abstract

Microbiologically influenced corrosion (MIC) constantly occurs in water/wastewater systems, especially in marine water. MIC contributes to billions of dollars in damage to marine industry each year, yet the physiological mechanisms behind this process remain poorly understood. Pseudomonas aeruginosa is a representative marine electro-active bacterium, which has been confirmed to cause severe MIC on carbon steel through extracellular electron transfer (EET). However, little is known about how P. aeruginosa causes corrosion on stainless steel. In this study, the corrosivity of wild-type strain, phzH knockout, phzH complemented, and phzH overexpression P. aeruginosa mutants were evaluated to explore the underlying MIC mechanism. We found the accelerated MIC on 2205 duplex stainless steel (DSS) was due to the secretion of phenazine-1-carboxamide (PCN), which was regulated by the phzH gene. Surface analysis, Mott-Schottky test and HO measurement results showed that PCN damaged the passive film by forming HO to oxidize chromium oxide to soluble hexavalent chromium, leading to more severe pitting corrosion. The normalized corrosion rate per cell followed the same order as the general corrosion rate obtained under each experimental condition, eliminating the influence of the total amount of sessile cells on corrosion. These findings provide new insight and are meaningful for the investigation of MIC mechanisms on stainless steel. The understanding of MIC can improve the sustainability and resilience of infrastructure, leading to huge environmental and economic benefits.

Address: Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China; School of Metallurgy, Northeastern University, Shenyang, China; Electrobiomaterials Institute, Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China.; Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China; Electrobiomaterials Institute, Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China.; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.; School of Metallurgy, Northeastern University, Shenyang, China.; Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China.; School of Civil, Mining and Environmental Engineering, University of Wollongong, Australia. Electronic address: [email protected].; Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, 110819, China; Electrobiomaterials Institute, Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang, 110819, China. Electronic address: [email protected].

DOI: 10.1016/j.watres.2022.118634

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0043...

MeSH Terms: Biofilms, Corrosion, Electrons, Hydrogen Peroxide, Pseudomonas aeruginosa, Stainless Steel, Steel, Water