Spatiotemporal monitoring of NAD metabolism with fluorescent biosensors.

Weicai Chen, Shuning Liu, Yi Yang, Zhuo Zhang, Yuzheng Zhao

Journal: Mechanisms of ageing and development 2022;204():111657

PMID: 35288098

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Abstract

Nicotinamide adenine dinucleotide (NAD) plays vital roles in cell metabolism, cell signaling, and gene expression regulation. NAD shows intricate subcellular distribution and dynamic changes in diverse biological processes; however, traditional biochemical assays usually require cell lysis, making it technically difficult to measure NAD metabolism in live cells or in vivo. Recently, a few genetically encoded and semisynthetic fluorescent sensors have been used to monitor NAD metabolism in a variety of biological settings. In this review, we summarize the recent progress in the development of fluorescent sensors for NAD and their applications in life science research. We further analyze their advantages, limitations, and perspectives for future development.

Address: Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.; Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.; Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China; Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing 100730, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China. Electronic address: [email protected].; Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China; Research Unit of New Techniques for Live-cell Metabolic Imaging, Chinese Academy of Medical Sciences, Beijing 100730, China; Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China. Electronic address: [email protected].

DOI: 10.1016/j.mad.2022.111657

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MeSH Terms: Biosensing Techniques, NAD