An inverse association between plasma benzoxazinoid metabolites and PSA after rye intake in men with prostate cancer revealed with a new method.

Elise Nordin, Stine K Steffensen, Bente B Laursen, Sven-Olof Andersson, Jan-Erik Johansson, Per Åman, Göran Hallmans, Michael Borre, Dan Stærk, Kati Hanhineva, Inge S Fomsgaard, Rikard Landberg

Journal: Scientific reports 2022;12(1):5260

PMID: 35347164

Free Full Text on Nature Publishing Group View on PubMed

Abstract

Prostate cancer (PC) is a common cancer among men, and preventive strategies are warranted. Benzoxazinoids (BXs) in rye have shown potential against PC in vitro but human studies are lacking. The aim was to establish a quantitative method for analysis of BXs and investigate their plasma levels after a whole grain/bran rye vs refined wheat intervention, as well as exploring their association with PSA, in men with PC. A quantitative method for analysis of 22 BXs, including novel metabolites identified by mass spectrometry and NMR, was established, and applied to plasma samples from a randomized crossover study where patients with indolent PC (n = 17) consumed 485 g whole grain rye/rye bran or fiber supplemented refined wheat daily for 6 wk. Most BXs were significantly higher in plasma after rye (0.3-19.4 nmol/L in plasma) vs. refined wheat (0.05-2.9 nmol/L) intake. HBOA-glc, 2-HHPAA, HBOA-glcA, 2-HPAA-glcA were inversely correlated to PSA in plasma (p < 0.04). To conclude, BXs in plasma, including metabolites not previously analyzed, were quantified. BX metabolites were significantly higher after rye vs refined wheat consumption. Four BX-related metabolites were inversely associated with PSA, which merits further investigation.

Address: Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 39, Gothenburg, Sweden. [email protected].; Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark. [email protected].; Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark.; Department of Urology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.; Department of Molecular Sciences, Swedish University of Agricultural Sciences, Box 7015, Uppsala, Sweden.; Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden.; Department of Urology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.; Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 39, Gothenburg, Sweden.; Department of Life Technologies, Food Chemistry and Food Development Unit, 20520, Turku, Finland.; School of Medicine, Institute of Public Health and Clinical Nutrition, University of Eastern Finland, 70210, Kuopio, Finland.; Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark. [email protected].; Division of Food and Nutrition Science, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 39, Gothenburg, Sweden. [email protected].; Department of Public Health and Clinical Medicine, Umeå University, Umeå, Sweden. [email protected].

DOI: 10.1038/s41598-022-08856-z

Link outs

Free resources

Full Text Sources:

Medical:

https://medlineplus.gov/prostatecancer.html

Miscellaneous:

https://assays.cancer.gov/CPTAC-415

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-PSAT1-1

MeSH Terms: Benzoxazines, Cross-Over Studies, Humans, Male, Prostate-Specific Antigen, Prostatic Neoplasms, Secale