Lateral flow immunoassay with peptide-functionalized gold nanoparticles for rapid detection of protein tyrosine phosphatase 1B.

Xiaotong Li, Qunyan Zhu, Fengqin Xu, Minghong Jian, Chaoqun Yao, Hua Zhang, Zhenxin Wang

Journal: Analytical biochemistry 2022;648():114671

PMID: 35367218

Full Text on Elsevier Science View on PubMed

Abstract

In this work, a lateral flow immunoassay (LFIA) with peptide functionalized gold nanoparticles (termed as biotin-peptide-AuNPs) has been developed for rapid, semi-quantitative detection of PTP1B activity without using any sophisticated equipment. In this method, the anti-phosphotyrosine (anti-pY) monoclonal antibody and streptavidin were used as test line and control line, respectively. The biotin-peptide-AuNPs contain 10% biotinylated peptide ligand carry a motif SDGHEpYIYVDP with pY (phosphotyrosine) and 90% pentapeptide (CALNN) ligand, which are used as PTP1B substrates and LFIA labelling probes. The experimental results demonstrate that the as-proposed LFIA with biotin-peptide-AuNPs exhibits a wide linear range (from 50 ng/mL to 10 μg/mL), a relatively low limit of detection (LOD, 44 ng/mL), and good specificity. In addition, the LFIA with biotin-peptide-AuNPs has been successfully used to evaluate activity levels of PTP1B in four cell lysates and the detection results exhibit a consistent trend with that of commercial kit.

Address: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Jinzhai Road Baohe District, Hefei, Anhui, 230026, China.; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Jinzhai Road Baohe District, Hefei, Anhui, 230026, China. Electronic address: [email protected].

DOI: 10.1016/j.ab.2022.114671

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MeSH Terms: Biotin, Gold, Immunoassay, Ligands, Limit of Detection, Metal Nanoparticles, Peptides, Protein Tyrosine Phosphatase, Non-Receptor Type 1