Identification of New QTLs for Dietary Fiber Content in .
Klaudia Kruppa, István Molnár, Agata Gadaleta, Jaroslav Doležel, Petr Cápal, Mahmoud Said, Éva Szakács, Éva Darkó, Péter Kovács, László Ivanizs, Eszter Gaál, Edina Türkösi, András Farkas, Kitti Szőke-Pázsi, Balázs Kalapos, Marianna Rakszegi, Ilaria Marcotuli
Journal: International journal of molecular sciences
2022;23(7):3821
PMID: 35409181
Abstract
["Grain dietary fiber content is an important health-promoting trait of bread wheat. A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass has high \u03b2-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition. In order to support introgression breeding, this work examined genetic variability in grain \u03b2-glucan, pentosan, and protein content in a collection of . A large variation in grain protein and edible fiber content was revealed, reflecting the origin of accessions from different eco-geographical habitats. Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with \u03b2-glucan, pentosan, water-extractable pentosan, and protein content. Mapping the markers to draft chromosome assemblies of diploid progenitors of underlined the role of genes on chromosomes 1M, 4M, and 5M in the formation of grain \u03b2-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content. Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots. The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods."]
Address:
Department of Biological Resources, Centre for Agricultural Research, Eötvös Loránd Research Network, Brunszvik u.2, 2462 Martonvásár, Hungary.; Department of Agricultural and Environmental Science, University of Bari 'Aldo Moro', Via G. Amendola 165/A, 70126 Bari, Italy.; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 779 00 Olomouc, Czech Republic.; Field Crops Research Institute, Agricultural Research Centre, 9 Gamma street-Giza, Cairo 12619, Egypt.