Study on synergistic effects of carboxymethyl cellulose and LIPUS for bone tissue engineering.

Liang Tang, Tianpei Wu, Yaling Zhou, Yi Zhong, Lijun Sun, Jianzhong Guo, Xiushan Fan, Dean Ta

Journal: Carbohydrate polymers 2022;286():119278

PMID: 35337527

Full Text on Elsevier Science View on PubMed

Abstract

In this research, the pharmaceutical used carboxymethyl cellulose (CMC) was successfully synthesized by ramie fiber and sodium monochloroacetate. Meanwhile, this study focused on the proliferation and differentiation of osteoblast which was stimulated by ramie based CMC. Additionally, the synergistic effects between CMC and Low-intensity pulsed ultrasound (LIPUS) for the proliferation and differentiation of osteoblast was also evaluated in this study. The experimental results demonstrated that ramie based CMC were nontoxic and cytocompatible in MC3T3-E1 cells culture; the dose of 300 μg L CMC applied in this work displayed better efficiency of promoting the osteogenic proliferation and differentiation. Meanwhile, the results were also exhibited that the combination of CMC and LIPUS (1.5 MHz, 80 mW cm) has a significant proliferation and differentiation promoting effects compared with the single factor intervention. This study provides a new pathway for bio-polymeric materials in the repair and regeneration of the bone tissues.

Address: Institute of Sports Biology, Shaanxi Normal University, Xi'an, China.; Shaanxi Key Laboratory of Ultrasonics, Shaanxi Normal University, Xi'an, China.; Institute of Sports Biology, Shaanxi Normal University, Xi'an, China. Electronic address: [email protected].; Department of Electronic Engineering, Fudan University, Shanghai, China; Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention (MICCAI) of Shanghai, Shanghai, China. Electronic address: [email protected].

DOI: 10.1016/j.carbpol.2022.119278

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MeSH Terms: Bone and Bones, Carboxymethylcellulose Sodium, Cell Differentiation, Osteogenesis, Tissue Engineering, Ultrasonic Waves