Quantitative Analysis of Posttranslational Modifications of Plant Histones.

Hana Kuchaříková, Zuzana Plšková, Zbyněk Zdráhal, Miloslava Fojtová, Pavel Kerchev, Gabriela Lochmanová

Journal: Methods in molecular biology (Clifton, N.J.) 2022;2526():241-257

PMID: 35657525

Abstract

Reshaping of the chromatin landscape under oxidative stress is of paramount importance for mounting an effective stress response. Unbiased systemic identification and quantification of histone marks is crucial for understanding the epigenetic component of plant responses to adverse environmental conditions. We describe a detailed method for isolation of plant histones and subsequent bottom-up proteomics approach for characterization of acetylation and methylation status. By performing label-free quantitative mass spectrometry analysis, relative abundances of histone marks can be statistically compared between experimental conditions.

Address: Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.; Phytophthora Research Centre, Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, Brno, Czech Republic.; Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic. [email protected].

DOI: 10.1007/978-1-0716-2469-2_18

Link outs

Subscription / membership required

Full Text Sources:

https://doi.org/10.1007/978-1-0716-2469-2_18

MeSH Terms: Acetylation, Histone Code, Histones, Methylation, Protein Processing, Post-Translational