Investigating Mitochondrial Ca Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters.

Gauri Bhosale, Michael R Duchen

Journal: Methods in molecular biology (Clifton, N.J.) 2022;2497():325-332

PMID: 35771454

Abstract

Mitochondrial Ca buffering is a hallmark of eukaryotic cellular physiology, contributing to the spatiotemporal shaping of the cytosolic Ca signals and regulation of mitochondrial bioenergetics. Often, this process is altered in a pathological context; therefore, it can be scrutinized experimentally for therapeutic intervention. In this chapter, we describe fluorescence and bioluminescence measurement of mitochondrial Ca in both isolated mitochondria and intact cells.

Address: Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK.; Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK. [email protected].

DOI: 10.1007/978-1-0716-2309-1_23

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https://doi.org/10.1007/978-1-0716-2309-1_23

MeSH Terms: Calcium, Calcium Signaling, Cytosol, Fluorescent Dyes, Mitochondria