Abstract
["The aboveground parts of plants are covered with cuticle, a hydrophobic layer composed of cutin polyester and cuticular wax that can protect plants from various environmental stresses. -Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme in plant wax synthesis. Although the properties of family genes have been investigated in many plant species, the understanding of this gene family in sorghum is still limited. Here, a total of 25 genes were identified in the sorghum genome, which were named from to . Evolutionary analysis among different species divided the family into five subfamilies and the SbKCSs were more closely related to maize, implying a closer evolutionary relationship between sorghum and maize. All genes were located on chromosomes 1, 2, 3, 4, 5, 6, 9 and 10, respectively, while Chr 1 and Chr 10 contained more genes than other chromosomes. The prediction results of subcellular localization showed that SbKCSs were mainly expressed in the plasma membrane and mitochondria. Gene structure analysis revealed that there was 0-1 intron in the sorghum family and within the same subgroup were similar. Multiple -acting elements related to abiotic stress, light and hormone response were enriched in the promoters of genes, which indicated the functional diversity among these genes. The three-dimensional structure analysis showed that a compact spherical space structure was formed by various secondary bonds to maintain the stability of SbKCS proteins, which was necessary for their biological activity. qRT-PCR results revealed that nine randomly selected genes expressed differently under drought and salt treatments, among which showed the greatest fold of expression difference at 12 h after drought and salt stresses, which suggested that the genes played a potential role in abiotic stress responses. Taken together, these results provided an insight into investigating the functions of family in sorghum and in response to abiotic stress.",{"copyright":"\u00a92022 Zhang et al."}]
