Sensitive electrochemical immunosensor for CYFRA21-1 detection based on AuNPs@MoS@TiCT composites.

Kai Hu, Jiamin Cheng, Kangbin Wang, Yuanqing Zhao, Yanju Liu, Huaixia Yang, Zhenqiang Zhang

Journal: Talanta 2021;238(Pt 1):122987

PMID: 34857321

Full Text on Elsevier Science View on PubMed

Abstract

Cytokeratin fragment antigen 21-1 (CYFRA21-1) is a sensitive marker for detecting non-small cell lung cancer (NSCLC). TiCT modified by gold nanoparticles (AuNPs) and molybdenum disulfide (MoS) were synthesized for the first time to obtain the AuNPs@MoS@TiCT composites, which have large specific surface area and good electrocatalytic properties. A novel electrochemical immunoassay for sensitive detection of CYFRA21-1 was developed by loading a large quantity of secondary antibodies (Ab) and toluidine blue (TB) on the surface of the material as signal probe, and Nafion-AuNPs mixture as electrode material. When the electrochemical response value of CYFRA21-1 increased linearly within the concentration range of 0.5 pg mL-50 ng mL, the detection limit can reach as low as 0.03 pg mL. In addition, the experimental results showed that the biosensor had the potential to rapidly detect CYFRA21-1 in the complex samples such as patient serum, and had a broad application prospect in the early diagnosis and monitoring of NSCLC.

Address: Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: [email protected].; Henan University of Chinese Medicine, Zhengzhou, 450046, PR China.; Henan Research Institute of Breeding Livestock and Poultry Industry Co., Ltd, Zhengzhou, 450000, PR China.; Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: [email protected].; Henan University of Chinese Medicine, Zhengzhou, 450046, PR China. Electronic address: [email protected].

DOI: 10.1016/j.talanta.2021.122987

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MeSH Terms: Antigens, Neoplasm, Biosensing Techniques, Carcinoma, Non-Small-Cell Lung, Electrochemical Techniques, Gold, Humans, Immunoassay, Keratin-19, Limit of Detection, Lung Neoplasms, Metal Nanoparticles, Molybdenum, Titanium