Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Paschalis-Thomas Doulias, Margarita Tenopoulou, Iordanis Zakopoulos, Harry Ischiropoulos

Journal: Nitric oxide : biology and chemistry 2022;117():1-6

PMID: 34536587

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Abstract

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical tips for the best performance of the experimental protocol.

Address: Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA; Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. Electronic address: [email protected].; Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA; Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.; Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA.; Children's Hospital of Philadelphia Research Institute, 3517 Civic Center Boulevard, Philadelphia, PA, 19104-4318, USA. Electronic address: [email protected].

DOI: 10.1016/j.niox.2021.09.004

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MeSH Terms: Chromatography, Cysteine, Mercury, Nitrates, Nitric Oxide, Nitrosation, Peptides, Proteins, Proteomics