Long-term impact of congenital toxoplasmosis on phenotypic and functional features of circulating leukocytes from infants one year after treatment onset.

Thádia Evelyn de Araújo, Angelica Oliveira Gomes, Jordana Grazziela Coelho-Dos-Reis, Ana Carolina Aguiar Vasconcelos Carneiro, Anderson Silva Machado, Gláucia Manzan Queiroz Andrade, Daniel Vitor Vasconcelos-Santos, José Nélio Januário, Vanessa Peruhype-Magalhães, Andréa Teixeira-Carvalho, Ricardo Wagner Almeida Vitor, Lis Ribeiro do Valle Antonelli, Eloisa Amalia Vieira Ferro, Olindo Assis Martins-Filho

Journal: Clinical immunology (Orlando, Fla.) 2021;232():108859

PMID: 34563685

Full Text on Elsevier Science View on PubMed

Abstract

Changes in immune response of children with congenital toxoplasmosis (CT) regarding infection evolution and therapeutic intervention was addressed. Infants with CT presented increased counts of monocytes, CD3CD16CD56, CD3CD56 and CD4 T-cells 1-year after treatment onset (TOXO). Smaller numbers of CD3CD16CD56 and TCRγδ T-cells were specifically observed in infants with retinochoroidal lesions (L(+)). When infants were classified based on the baseline status, expansion of CD3CD16CD56 and CD4 T-cells were observed in L(+) who had active, active/cicatricial or cicatricial lesions. Infants who had active or active/cicatricial lesions display augmented numbers of monocytes, CD3CD16CD56, CD3CD56, CD8DR and TCRγδ T-cells and those with active/cicatricial or cicatricial at baseline displayed increase in CD14CD64 monocytes. Moreover, all L(+) had increased IFN-γ and IL-10 CD4 T-cells, while L(-) had increased ratios of TNF, IFN-γ and IL-4 NK-cells upon antigen-specific stimulation. Persistent alterations in leukocytes in TOXO suggest long-term sequels in the immune system of infants with CT.

Address: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil; Instituto René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil. Electronic address: [email protected].; Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Rua Frei Paulino, 30, Nossa Sra. da Abadia, 38025-180 Uberaba, MG, Brazil.; Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos, 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.; Departamento de Pediatria, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil; Núcleo de Ações e Pesquisa em Apoio Diagnóstico (NUPAD), Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil.; Núcleo de Ações e Pesquisa em Apoio Diagnóstico (NUPAD), Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil; Departamento de Oftalmologia e Otorrinolaringologia, Faculdade de Medicina, UFMG, Belo Horizonte, MG, Brazil.; Núcleo de Ações e Pesquisa em Apoio Diagnóstico (NUPAD), Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil.; Instituto René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.; Instituto René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil. Electronic address: [email protected].; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil.; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Avenida João Naves de Ávila 2121, Santa Mônica, 38408-100 Uberlândia, MG, Brazil; Instituto René Rachou, Fundação Oswaldo Cruz, Avenida Augusto de Lima, 1715, Barro Preto, 30190-002 Belo Horizonte, MG, Brazil.; Uveitis Unit, Hospital Sao Geraldo, Hospital das Clínicas, UFMG, Brazil.; Departamento de Pediatria, Universidade Federal de Minas Gerais, Avenida Professor Alfredo Balena 190, Santa Efigênia, 30130-100 Belo Horizonte, MG, Brazil.; Department of Phonoaudiology, School of Medicine, UFMG, Brazil.

DOI: 10.1016/j.clim.2021.108859

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MeSH Terms: Antiprotozoal Agents, Female, Humans, Infant, Infant, Newborn, Lymphocytes, Male, Monocytes, Phenotype, Pyrimethamine, Sulfadiazine, Time, Toxoplasmosis, Congenital