Remodelling of Ca homeostasis is linked to enlarged endoplasmic reticulum in secretory cells.

Tillman Pick, Andreas Beck, Igor Gamayun, Yvonne Schwarz, Claudia Schirra, Martin Jung, Elmar Krause, Barbara A Niemeyer, Richard Zimmermann, Sven Lang, Eelco van Anken, Adolfo Cavalié

Journal: Cell calcium 2022;99():102473

PMID: 34560367

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Abstract

The endoplasmic reticulum (ER) is extensively remodelled during the development of professional secretory cells to cope with high protein production. Since ER is the principal Ca store in the cell, we characterised the Ca homeostasis in NALM-6 and RPMI 8226 cells, which are commonly used as human pre-B and antibody secreting plasma cell models, respectively. Expression levels of Sec61 translocons and the corresponding Sec61-mediated Ca leak from ER, Ca storage capacity and store-operated Ca entry were significantly enlarged in the secretory RPMI 8226 cell line. Using an immunoglobulin M heavy chain producing HeLa cell model, we found that the enlarged Ca storage capacity and Ca leak from ER are linked to ER expansion. Our data delineates a developmental remodelling of Ca homeostasis in professional secretory cells in which a high Sec61-mediated Ca leak and, thus, a high Ca turnover in the ER is backed up by enhanced store-operated Ca entry.

Address: Experimental and Clinical Pharmacology and Toxicology, Pre-clinical Center for Molecular Signalling (PZMS), Saarland University, 66421 Homburg, Germany. Electronic address: [email protected].; Experimental and Clinical Pharmacology and Toxicology, Pre-clinical Center for Molecular Signalling (PZMS), Saarland University, 66421 Homburg, Germany.; Molecular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, 66421 Homburg, Germany.; Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, 66421 Homburg, Germany.; Medical Biochemistry and Molecular Biology, Pre-clinical Centre for Molecular Signalling (PZMS), Saarland University, 66421 Homburg, Germany.; Molecular Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, 66421 Homburg, Germany.; Division of Genetics and Cell Biology, San Raffaele Scientific Institute and Università Vita-Salute San Raffaele, Milan, Italy.; Experimental and Clinical Pharmacology and Toxicology, Pre-clinical Center for Molecular Signalling (PZMS), Saarland University, 66421 Homburg, Germany. Electronic address: [email protected].

DOI: 10.1016/j.ceca.2021.102473

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MeSH Terms: Calcium, Calcium Signaling, Endoplasmic Reticulum, HeLa Cells, Homeostasis, Humans, SEC Translocation Channels