Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum.

Luca Fiore, Benedetta De Lellis, Vincenzo Mazzaracchio, Elena Suprun, Renato Massoud, Bianca Maria Goffredo, Danila Moscone, Fabiana Arduini

Journal: Talanta 2021;237():122869

PMID: 34736707

Full Text on Elsevier Science View on PubMed

Abstract

Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 μM, with a detection limit equal to 4.4 μM. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples.

Address: Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133, Rome, Italy.; Institute of Biomedical Chemistry, Pogodinskaya Street, 10/8, 119121, Moscow, Russia.; Department of Experimental Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133, Rome, Italy.; Metabolic Pathology Laboratory, I.R.C.C.S. Bambino Gesù Pediatric Hospital, Via Ferdinando Baldelli 38, Rome, Italy.; Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133, Rome, Italy; SENSE4MED, Via Renato Rascel 30, 00128, Rome, Italy. Electronic address: [email protected].

DOI: 10.1016/j.talanta.2021.122869

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MeSH Terms: Electrochemical Techniques, Electrodes, Limit of Detection, Smartphone, Soot, Tyrosine