Bacterial strains found in the soils of a municipal solid waste dumping site facilitated phosphate solubilization along with cadmium remediation.

Sudha Sahu, Manas Protim Rajbonshi, Nihal Gujre, Manish Kumar Gupta, Rahul G Shelke, Anamika Ghose, Latha Rangan, Kannan Pakshirajan, Sudip Mitra

Journal: Chemosphere 2021;287(Pt 3):132320

PMID: 34826951

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Abstract

Phosphate solubilizing bacteria (PSB) that can withstand high cadmium (Cd) stress is a desired combination for bioremediation. This study evaluated the Cd bioremediation potential of four PSB strains isolated from the contaminated soils of a municipal solid waste (MSW) discarding site (Guwahati, India). PSB strains were cultured in Pikovskaya (PVK) media, which led to higher acid phosphatase (ACP) activity and the release of organic acid. Optical density (OD) measurements were performed to determine the growth pattern of PSB; furthermore, Cd uptake by PSB was evaluated using infrared spectroscopy (IR) and X-Ray Diffraction (XRD) analyses. The 16S rRNA taxonomic analysis revealed that all the four promising PSB strains belonged to either Bacillus sp. or Enterobacter sp. One strain (SM_SS8) demonstrated higher tolerance towards Cd (up to 100 mg L). Flow cytometry analysis revealed 70.92%, 46.93% and 20.4% viability of SM_SS8 in 10, 50 and 100 mg L, respectively in PVK media containing Cd. This study has therefore substantiated the bioremediation of Cd from polluted soil by the PSB isolates. Thus, experimental results revealed a potential combo benefit, phosphate solubilization along with Cd remediation.

Address: Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India; Department of Zoology, Government Kamla Nehru Girls College, Balaghat, Madhya Pradesh, 481001, India.; Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India.; Applied Biodiversity Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India.; Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India; Applied Biodiversity Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India.; Agro-ecotechnology Laboratory, School of Agro and Rural Technology, Indian Institute of Technology Guwahati, Assam, 781039, India. Electronic address: [email protected].

DOI: 10.1016/j.chemosphere.2021.132320

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MeSH Terms: Bacteria, Biodegradation, Environmental, Cadmium, Phosphates, RNA, Ribosomal, 16S, Soil, Soil Microbiology, Soil Pollutants, Waste Disposal Facilities