Exogenous sodium fumarate enhances astaxanthin accumulation in Haematococcus pluvialis by enhancing the respiratory metabolic pathway.

Wenjie Yu, Litao Zhang, Jing Zhao, Jianguo Liu

Journal: Bioresource technology 2021;341():125788

PMID: 34461402

Full Text on Elsevier Science View on PubMed

Abstract

Improvement of astaxanthin yield is a continuing objective in Haematococcus pluvialis cultivation. In this study, a new strategy combining exogenous sodium fumarate (SF) with nitrogen deficiency was demonstrated to promote astaxanthin accumulation in H. pluvialis. SF significantly accelerated astaxanthin synthesis over cell growth. Notably, under nitrogen deficiency, 10 mM SF increased the cellular astaxanthin content by 1.75-fold and 1.47-fold on day 7 and 12, respectively. Further studies indicated that SF increased the level of respiratory metabolites in the Embden-Meyerhof-Parnas pathway, tricarboxylic acid cycle and pentose phosphate pathway. An enhanced substrate pool due to the acceleration of respiratory metabolic pathways directly induced astaxanthin synthesis. Meanwhile, the accumulation of C18:1, C18:2 and C18:3 fatty acids enhanced astaxanthin synthesis indirectly by promoting the process of esterification. This study not only helps to elucidate the role of respiratory metabolic pathways in astaxanthin synthesis but also provides a new effective technique to improve astaxanthin production.

Address: Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; University of Chinese Academy of Sciences, Beijing 100049, China.; Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.; Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.; Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. Electronic address: [email protected].

DOI: 10.1016/j.biortech.2021.125788

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MeSH Terms: Chlorophyceae, Fumarates, Metabolic Networks and Pathways, Xanthophylls