Pontivivens ytuae sp. nov., isolated from deep sea sediment of the Mariana Trench.

A Gram-stain-negative, light pink-coloured, rod-shaped, flagellated and facultative anaerobic bacterial strain, designated MT2928^T, was isolated from deep-sea sediment collected from the Mariana Trench. Growth of strain MT2928^T occurred optimally at 28 °C, pH 8.0-9.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MT2928^T belongs to the genus Pontivivens and has the highest sequence similarity to Pontivivens insulae GYSW-23^T (96.6 %). Genomic analysis indicated that strain MT2928^T contains a circular chromosome of 4 199 362 bp with G+C content of 67.2 mol%. The strain did not produce bacteriochlorophyll a, but produced carotenoid. The predominant respiratory quinone of MT2928^T was ubiquinone-10. The polar lipids of MT2928^T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified lipids and two unidentified phospholipids. The major fatty acids of strain MT2928^T contained summed feature 8 (C_18 : 1  ω7c or/and C_18 : 1  ω6c), C_18 : 0 and summed feature 2 (iso-C_16 : 1 I and/or C_14 : 0 3-OH). On the basis of phylogenetic, physiological, biochemical and other phenotypic properties, strain MT2928^T represents a novel species of the genus Pontivivens, and the name Pontivivens ytuae sp. nov. is proposed with the type species MT2928^T (=MCCC 1K05575^T=JCM 34320^T).