SERS liquid biopsy: An emerging tool for medical diagnosis.

Vlad Moisoiu, Stefania D Iancu, Andrei Stefancu, Tudor Moisoiu, Barbara Pardini, Mihnea P Dragomir, Nicolae Crisan, Lucretia Avram, Dana Crisan, Iulia Andras, Daniela Fodor, Loredana F Leopold, Carmen Socaciu, Zoltán Bálint, Ciprian Tomuleasa, Florin Elec, Nicolae Leopold

Journal: Colloids and surfaces. B, Biointerfaces 2021;208():112064

PMID: 34517219

Full Text on Elsevier Science View on PubMed

Abstract

Surface-enhanced Raman scattering (SERS) is emerging as a novel strategy for biofluid analysis. In this review, we delineate four experimental SERS protocols that are frequently used for the profiling of biofluids: 1) liquid SERS for the detection of purine metabolites; 2) iodide-modified liquid SERS for the detection of proteins; 3) dried SERS for the detection of both purine metabolites and proteins; 4) resonant Raman for the detection of carotenoids. To explain the selectivity of each experimental SERS protocol, we introduce a heuristic model for the chemisorption of analytes mediated by adsorbed ions (adions) onto the SERS substrate. Next, we show that the promising results of SERS liquid biopsy stem from the fact that the concentration levels of purine metabolites, proteins and carotenoids are informative of the cellular turnover rate, inflammation, and oxidative stress, respectively. These processes are perturbed in virtually every disease, from cancer to autoimmune maladies. Finally, we review recent SERS liquid biopsy studies and discuss future steps that are required for translating SERS in the clinical setting.

Address: Faculty of Physics, Babeș-Bolyai University, 400084, Cluj-Napoca, Romania.; Clinical Institute of Urology and Renal Transplant, 400006, Cluj-Napoca, Romania; Biomed Data Analytics SRL, 400696, Cluj-Napoca, Romania; Department of Urology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania.; Candiolo Cancer Institute, FPO-IRCCS, 10060, Candiolo, Italy; Italian Institute of Genomic Medicine (IIGM), 10060, Candiolo, Italy.; Institute of Pathology, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 10117, Berlin, Germany.; Department of Urology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania; Clinical Municipal Hospital, 400139, Cluj-Napoca, Romania.; Clinical Municipal Hospital, 400139, Cluj-Napoca, Romania; Department of Geriatrics, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania.; Clinical Municipal Hospital, 400139, Cluj-Napoca, Romania; 5th Internal Medicine Department, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania.; 2nd Internal Medicine Department, Iuliu Hatieganu University of Medicine and Pharmacy, 400006, Cluj-Napoca, Romania.; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj-Napoca, Romania.; Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372, Cluj-Napoca, Romania; BIODIATECH Research Centre for Applied Biotechnology, SC Proplanta, 400478, Cluj-Napoca, Romania.; Department of Hematology, Iuliu Hatieganu University of Medicine and Pharmacy, 400124, Cluj-Napoca, Romania; Department of Hematology, Ion Chiricuta Clinical Cancer Center, 400124, Cluj-Napoca, Romania; Medfuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400349, Cluj-Napoca, Romania.; Clinical Institute of Urology and Renal Transplant, 400006, Cluj-Napoca, Romania; Department of Urology, Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania. Electronic address: [email protected].; Faculty of Physics, Babeș-Bolyai University, 400084, Cluj-Napoca, Romania; Biomed Data Analytics SRL, 400696, Cluj-Napoca, Romania. Electronic address: [email protected].

DOI: 10.1016/j.colsurfb.2021.112064

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MeSH Terms: Humans, Liquid Biopsy, Neoplasms, Proteins, Spectrum Analysis, Raman