Mitochondria-targeted acridine-based dual-channel fluorescence chemosensor for detection of Sn and CrO ions in water and its application in discriminative detection of cancer cells.

Palanisamy Ravichandiran, D S Prabakaran, Nikhil Maroli, Ae Rhan Kim, Byung-Hyun Park, Myung-Kwan Han, Thiyagarajan Ramesh, Samuel Ponpandian, Dong Jin Yoo

Journal: Journal of hazardous materials 2021;419():126409

PMID: 34171666

Full Text on Elsevier Science View on PubMed

Abstract

The goal of the present work was to fabricate a new low-cost, easy-to-prepare, dual-channel fluorescence chemosensor comprised of acridine-diphenylacetyl moieties (NDA) to enable remarkable Sn detection in water and biological medium. The resulting NDA-Sn complex was utilized for the distinguished identification of CrO ions from other anions and biomolecules. These investigations involve the absorption, fluorescence, and electrochemical methods for the detection of Sn and CrO ions in pure water. The mechanism for NDA-mediated Sn detection was experimentally determined by FT-IR, NMR titrations, mass (ESI) analyses, and DFT calculations. The obtained results indicate that the NDA chemosensor possessed excellent performance characteristics including good water solubility and compatibility, quick response time (less than 10 s), high sensitivity (Sn = 0.268 μM and CrO = 0.160 μM), and selectivity against coexisting metals, anions, amino acids, and peptides. The chemosensor NDA induced negligible toxicity in live cells and was successfully utilized as a biomarker for the tracking of Sn in human normal and cancer cells. More importantly, NDA demonstrates distinguished recognition of Sn in human cancer cells rather than in normal live cells. Additionally, NDA was shown to act as a mitochondria-targeted probe in FaDu cells.

Address: R&D Education Center for Whole Life Cycle R&D of Fuel Cell Systems, Jeonbuk National University, Jeonju, Jeollabuk-do 54896, Republic of Korea; Department of Life Science, Department of Energy Storage/Conversion Engineering of Graduate School, Hydrogen and Fuel Cell Research Center, Jeonbuk National University, Jeonju, Jeollabuk-do 54896, Republic of Korea. Electronic address: [email protected].; Department of Radiation Oncology, College of Medicine, Chungbuk National University, Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk 28644, Republic of Korea; Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Srivilliputhur Main Road, Sivakasi 626124, Tamil Nadu, India.; Center for Condensed Matter Theory, Department of Physics, Indian Institute of Science, Bangalore 560012, India.; Department of Life Science, Department of Energy Storage/Conversion Engineering of Graduate School, Hydrogen and Fuel Cell Research Center, Jeonbuk National University, Jeonju, Jeollabuk-do 54896, Republic of Korea.; Department of Biochemistry, Jeonbuk National University Medical School, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.; Department of Microbiology, Jeonbuk National University Medical School, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.; Department of Basic Medical Sciences, College of Medicine, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.; Department of Biotechnology, Ayya Nadar Janaki Ammal College (Autonomous), Sivakasi, Srivilliputhur Main Road, Sivakasi 626124, Tamil Nadu, India.; R&D Education Center for Whole Life Cycle R&D of Fuel Cell Systems, Jeonbuk National University, Jeonju, Jeollabuk-do 54896, Republic of Korea; Department of Life Science, Department of Energy Storage/Conversion Engineering of Graduate School, Hydrogen and Fuel Cell Research Center, Jeonbuk National University, Jeonju, Jeollabuk-do 54896, Republic of Korea. Electronic address: [email protected].

DOI: 10.1016/j.jhazmat.2021.126409

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MeSH Terms: Acridines, Fluorescent Dyes, Humans, Ions, Mitochondria, Neoplasms, Spectrometry, Fluorescence, Spectroscopy, Fourier Transform Infrared, Water