Lipase - The fascinating dynamics of enzyme in seed storage and germination - A real challenge to pearl millet.

Ranjeet R Kumar, D V Bhargava, Kangkan Pandit, Suneha Goswami, S Mukesh Shankar, Sumer P Singh, Gyanendra K Rai, C Tara Satyavathi, Shelly Praveen

Journal: Food chemistry 2021;361():130031

PMID: 34058661

Full Text on Elsevier Science View on PubMed

Abstract

Pearl millet is considered as 'nutri-cereal' because of high nutrient density of the seeds. The grain has limited use because of low keeping quality of the flour due to the activities of rancidity causing enzymes like lipase, lox, pox and PPO. Among all the enzymes, lipase is most notorious because of its robust nature and high activity under different conditions. we have identified 2180 putative transcripts showing homology with different variants of lipase precursor through transcriptome data mining (NCBI BioProject acc. no. PRJNA625418). Lipase plays dual role of facilitating the germination of seeds and deteriorating the quality of the pearl millet flour through hydrolytic rancidity. Different physiochemical methods like heat treatment, micro oven, hydrothermal, etc. have been developed to inhibit lipase activity in pearl millet flour. There is further need to develop improved processing technologies to inhibit the hydrolytic and oxidative rancidity in the floor with enhanced shelf-life.

Address: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi Pin 110012, India. Electronic address: [email protected].; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi Pin 110012, India.; Division of Genetics, Indian Agricultural Research Institute, New Delhi Pin 110012, India.; Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Pin 180009, India.; All India Coordinated Research Project on Pearl Millet, Jodhpur, Rajasthan Pin 342304, India.; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi Pin 110012, India. Electronic address: [email protected].

DOI: 10.1016/j.foodchem.2021.130031

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0308...

MeSH Terms: Flour, Food Handling, Food Storage, Germination, Lipase, Pennisetum, Seeds