Bioassay-Guided Identification of the Antiproliferative Compounds of and the Transcriptomic Effect of Resveratrol in Prostate Cancer Pc3 Cells.
Luis Fernando Méndez-López, Pierluigi Caboni, Eder Arredondo-Espinoza, Juan J J Carrizales-Castillo, Isaías Balderas-Rentería, María Del Rayo Camacho-Corona
Journal: Molecules (Basel, Switzerland)
2021;26(8):2200
PMID: 33920405
Abstract
["The bioassay-guided fractionation of a CHCl-MeOH extract from the stems of identified an active fraction against PC3 prostate cancer cells. The treatment for 24 h showed an 80% reduction in cell viability ( \u2264 0.05) by a WST-1 assay at a concentration of 100 \u03bcg\/mL. The HPLC-QTOF-MS analysis of the fraction showed the presence of coumaric and isoferulic acids, apigenin, kaempferol, chrysoeriol, naringenin, ursolic and betulinic acids, hexadecadienoic and octadecadienoic fatty acids, and the stilbene resveratrol. The exposure of PC3 cells to resveratrol (IC = 23 \u03bcg\/mL) for 24 h induced significant changes in 847 genes (Z-score \u2265 \u00b12). The functional classification tool of the DAVID v6.8 platform indicates that the underlying molecular mechanisms against the proliferation of PC3 cells were associated ( \u2264 0.05) with the process of differentiation and metabolism. These findings provide experimental evidence suggesting the potential of as a promising natural source of anticancer compounds."]
Address:
Laboratorio de Química Farmacéutica, Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, Ciudad Universitaria, San Nicolás de los Garza, Nuevo León 66451, Mexico.; Centro de Investigación en Nutrición y Salud Publica, Facultad de Salud Pública y Nutrición, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León 66460, Mexico.; Dipartamento Scienze della vita e dell'ambiente, Università degli Studi di Cagliari, Cittadella Universitaria, Monserrato, 09042 Cagliari, Italy.
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MeSH Terms:
Antineoplastic Agents, Phytogenic,
Apigenin,
Biological Assay,
Cell Proliferation,
Cell Survival,
Cissus,
Flavanones,
Flavones,
Gene Expression Profiling,
Humans,
Kaempferols,
Male,
Microarray Analysis,
Neoplasm Proteins,
PC-3 Cells,
Pentacyclic Triterpenes,
Plant Extracts,
Resveratrol,
Transcriptome,
Betulinic Acid
