Marine associated microbial consortium applied to RBBR textile dye detoxification and decolorization: Combined approach and metatranscriptomic analysis.

Gabriela Alves Licursi Vieira, Lucélia Cabral, Igor Vinicius Ramos Otero, Milene Ferro, Adriano Uemura de Faria, Valéria Maia de Oliveira, Mauricio Bacci, Lara Durães Sette

Journal: Chemosphere 2021;267():129190

PMID: 33316621

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Abstract

The combination of different microorganisms and their metabolisms makes the use of microbial consortia in bioremediation processes a useful approach. In this sense, this study aimed at structuring and selecting a marine microbial consortium for Remazol Brilliant Blue R (RBBR) detoxification and decolorization. Experimental design was applied to improve the culture conditions, and metatranscriptomic analysis to understand the enzymatic pathways. A promising consortium composed of Mucor racemosus CBMAI 847, Marasmiellus sp. CBMAI 1062, Bacillus subtilis CBMAI 707, and Dietzia maris CBMAI 705 was selected. This consortium showed 52% of detoxification and 86% of decolorization in the validation assays after seven days of incubation in the presence of 500 ppm of RBBR. Reduction in RBBR color and toxicity were achieved by biosorption and microbial metabolisms. Metatranscriptomic data indicate that the consortium was able to decolorize and breakdown the RBBR molecule using a coordinated action of oxidases, oxygenases, and hydrolases. Epoxide hydrolases and glyoxalases expression could be associated with the decrease in toxicity. The efficiency of this marine microbial consortium suggests their use in bioremediation processes of textile effluents.

Address: Departamento de Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].; Departamento de Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil.; Departamento de Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].; Centro de Estudos de Insetos Sociais, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].; Departamento de Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].; Universidade Estadual de Campinas (UNICAMP), Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, Divisão de Recursos Microbianos, Campinas, SP, Brazil. Electronic address: [email protected].; Centro de Estudos de Insetos Sociais, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].; Departamento de Biologia Geral e Aplicada, Instituto de Biociências, Universidade Estadual Paulista (UNESP), Rio Claro, SP, Brazil. Electronic address: [email protected].

DOI: 10.1016/j.chemosphere.2020.129190

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MeSH Terms: Actinobacteria, Biodegradation, Environmental, Coloring Agents, Microbial Consortia, Mucor, Textiles