The biochemical and genetic discovery of the SAGA complex.

Patrick A Grant, Fred Winston, Shelley L Berger

Journal: Biochimica et biophysica acta. Gene regulatory mechanisms 2021;1864(2):194669

PMID: 33338653

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Abstract

One of the major advances in our understanding of gene regulation in eukaryotes was the discovery of factors that regulate transcription by controlling chromatin structure. Prominent among these discoveries was the demonstration that Gcn5 is a histone acetyltransferase, establishing a direct connection between transcriptional activation and histone acetylation. This breakthrough was soon followed by the purification of a protein complex that contains Gcn5, the SAGA complex. In this article, we review the early genetic and biochemical experiments that led to the discovery of SAGA and the elucidation of its multiple activities.

Address: Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL 33431, United States of America.; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, United States of America. Electronic address: [email protected].; Department of Cell and Developmental Biology, Penn Epigenetics Institute, Department of Biology, Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States of America.

DOI: 10.1016/j.bbagrm.2020.194669

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MeSH Terms: Acetylation, Chromatin, Enzyme Assays, Histones, Humans, Protein Processing, Post-Translational, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, TATA-Box Binding Protein, Trans-Activators, Transcriptional Activation, Ubiquitination, p300-CBP Transcription Factors