Biochemistry and molecular genetics of poly-gamma-glutamate synthesis.

M Ashiuchi, H Misono

Journal: Applied microbiology and biotechnology 2002;59(1):9-14

PMID: 12073126

Abstract

Current research into poly-gamma-glutamate (PGA) and its biosynthesis is reviewed. In PGA-producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode the membrane-associated PGA synthetase complex PgsBCA, were characterized and the enzyme complex was suggested to be an atypical amide ligase based on its structure and function. A novel reaction mechanism of PGA synthesis is proposed.

Address: Department of Bioresources Science, Kochi University, Nankoku, Kochi, 783-8502, Japan. [email protected]

DOI: 10.1007/s00253-002-0984-x

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https://dx.doi.org/10.1007/s00253-002-0984-x

MeSH Terms: Amino Acid Isomerases, Bacillus subtilis, Cloning, Molecular, Glutamate Synthase, Glutamic Acid, Isoenzymes, Kinetics, Multienzyme Complexes, Polyglutamic Acid, Stereoisomerism