Cloning and expression of gene in two varieties.

is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The fulllength cDNAs of in conventional Lonicera macranthoides () and the mutant '' cultivar () were obtained using rapid amplification of cDNA ends and the expression vectors for were constructed to investigate the roles of in the cultivar. (GenBank: MK419948) and (GenBank: MK419948) were isolated from the conventional variety and '' cultivar respectively. is 1274 bp in length, whereas is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_like domain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerase chain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the genes were also expressed in stems and leaves. is underexpressed at flowering period 5 that the key time node for corolla expansion and nonexpansion, while is overexpressed during this flowering period. was speculated to be a functional gene causing different phenotypes in the two varieties. The successfully constructed plant expression vector provides an experimental reference for further research on the function of this gene and the basis for the excellent phenotype of ''.