Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State.

Petra Riegerová, Jindřich Brejcha, Dagmar Bezděková, Tomáš Chum, Eva Mašínová, Nikola Čermáková, Saak V Ovsepian, Marek Cebecauer, Martin Štefl

Journal: Journal of Alzheimer's disease : JAD 2021;82(2):485-491

PMID: 34057078

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Abstract

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.

Address: Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic.; Department of Biophysical Chemistry, J. Heyrovský Institute of Physical Chemistry of the Czech Academy of Sciences, Prague, Czech Republic.; Department of Philosophy and History of Science, Faculty of Science, Charles University, Prague, Czech Republic.; Department of Experimental Neurobiology, National Institute of Mental Health, Klecany, Czech Republic.; Department of Psychiatry and Medical Psychology, Third Faculty of Medicine, Charles University, Prague, Czech Republic.

DOI: 10.3233/JAD-201409

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MeSH Terms: Alzheimer Disease, Amyloid beta-Protein Precursor, Brain-Derived Neurotrophic Factor, Cell Differentiation, Cell Line, Tumor, Humans, Intravital Microscopy, Models, Biological, Neuroblastoma, Neurons, Oxidative Stress, Proteolysis, Tretinoin