Solid-State, Thermal Synthesis of Peptide/Protein-Boron Cluster Conjugates.

Krzysztof Fink, Tomasz M Goszczyński

Journal: Methods in molecular biology (Clifton, N.J.) 2022;2355():93-104

PMID: 34386953

Abstract

Anionic boron clusters can be used to increase the pharmaceutical properties of the peptides. Here, we describe the method of synthesis of peptide/protein-boron cluster conjugates using solid-state, thermal reaction on two different peptides: thymosin β4 (Tβ4) and lysozyme. 1,4-dioxane oxonium derivatives of anionic boron clusters are used as donors of boron clusters. This procedure allows to conjugate anionic boron clusters to native peptides without loss of the activity of the peptides.

Address: Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.; Laboratory of Biomedical Chemistry, Department of Experimental Oncology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. [email protected].

DOI: 10.1007/978-1-0716-1617-8_9

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https://doi.org/10.1007/978-1-0716-1617-8_9

MeSH Terms: Anions, Boron, Peptides, Thymosin