Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results.

Lisa Giovannelli, Sabine A S Langie, Alena Gabelova, Monika Sramkova, Vanessa Moraes de Andrade, Sona Vodenkova, Pavel Vodicka, Helga Stopper, Matjaz Novak, Bojana Zegura, Cristian Del Bo', Nursen Basaran, Vanessa Valdiglesias, Blanca Laffon, Elke Richling, Marcus S Cooke, Elisa Boutet-Robinet, Kristine B Gutzkow, Gunnar Brunborg, Roger Godschalk, Maria Dusinska, Cristiana Costa Pereira, João Paulo Teixeira, Solange Costa, Goran Gajski, Mirta Milić, Stefano Bonassi, Gudrun Koppen, Amaya Azqueta, Peter Møller, Andrew Collins

Journal: Nature protocols 2021;15(12):3817-3826

PMID: 33106678

Abstract

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

Address: Department of Public Health, Section of Environmental Health, University of Copenhagen, Copenhagen, Denmark. [email protected].; Department of Pharmacology and Toxicology, University of Navarra, Pamplona, Spain.; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain.; Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France.; VITO-HEALTH, Mol, Belgium.; Unit of Clinical and Molecular Epidemiology IRCCS San Raffaele Pisana, Rome, Italy.; Department of Human Sciences and Quality of Life Promotion, San Raffaele University, Rome, Italy.; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia.; Environmental Health Department, National Institute of Health Dr Ricardo Jorge, Porto, Portugal.; Epidemiological Research Unit (EPIUnit), Instituto de Saúde Pública, Universidade do Porto, Porto, Portugal.; Health Effects Laboratory, Department of Environmental Chemistry, Norwegian Institute for Air Research (NILU), Kjeller, Norway.; Department of Pharmacology & Toxicology, School for Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, Maastricht, the Netherlands.; Section of Molecular Toxicology, Department of Environmental Health, Norwegian Institute of Public Health Skøyen, Oslo, Norway.; Department of Neuroscience, Psychology, Pharmacology and Child Health (NEUROFARBA), Section Pharmacology and Toxicology, University of Florence, Florence, Italy.; Oxidative Stress Group, Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, FL, USA.; Food Chemistry & Toxicology, Department of Chemistry, University of Kaiserslautern, Kaiserslautern, Germany.; Universidade da Coruña, Grupo DICOMOSA, Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Psicología, Facultad de Ciencias de la Educación, Coruña, Spain.; Instituto de Investigación Biomédica de A Coruña (INIBIC), AE CICA-INIBIC, Coruña, Spain.; Department of Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.; Division of Human Nutrition, Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Milan, Italy.; Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia.; Institute of Pharmacology and Toxicology, University of Würzburg, Würzburg, Germany.; Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic.; Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Prague, Czech Republic.; Laboratory of Translational Biomedicine, University of Southern Santa Catarina, UNESC, Criciúma, Brazil.; Department of Nanobiology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Bratislava, Slovakia.; Department of Nutrition, University of Oslo, Oslo, Norway.; Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium.
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