Lisa Giovannelli, Sabine A S Langie, Alena Gabelova, Monika Sramkova, Vanessa Moraes de Andrade, Sona Vodenkova, Pavel Vodicka, Helga Stopper, Matjaz Novak, Bojana Zegura, Cristian Del Bo', Nursen Basaran, Vanessa Valdiglesias, Blanca Laffon, Elke Richling, Marcus S Cooke, Elisa Boutet-Robinet, Kristine B Gutzkow, Gunnar Brunborg, Roger Godschalk, Maria Dusinska, Cristiana Costa Pereira, João Paulo Teixeira, Solange Costa, Goran Gajski, Mirta Milić, Stefano Bonassi, Gudrun Koppen, Amaya Azqueta, Peter Møller, Andrew Collins
Journal: Nature protocols 2021;15(12):3817-3826
PMID: 33106678
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between 'desirable' and 'essential' information: 'essential' information refers to the precise details that are necessary to assess the quality of the experimental work, whereas 'desirable' information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
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