Modelling folates reaction kinetics during cowpea seed germination in comparison with soaking.

Fanny Coffigniez, Michael Rychlik, Christian Mestres, Lisa Striegel, Philippe Bohuon, Aurélien Briffaz

Journal: Food chemistry 2020;340():127960

PMID: 32916403

Full Text on Elsevier Science View on PubMed

Abstract

Folate is a fundamental vitamin for metabolism in plants and humans. A modelling approach has been developed to characterize the reactivity of folates in cowpea seeds during germination at 30 °C, using a water-to-seed ratio of 1:1 (w/w). For this purpose, the concentrations of folic acid, 10-formylfolic acid, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tetrahydrofolate were determined in seeds during germination times up to 96 h. Two reaction models were sequentially built and adjusted to experimental data to describe changes in concentration in cowpea seed during two germination phases: before 14 h and after 48 h. Results showed intense enzymatic interconversion of all folate vitamers into 5-methyltetrahydrofolate before 14 h of germination and high enzymatic production of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tetrahydrofolate after 48 h of germination. This study suggests that a long germination process could be more beneficial than soaking to increase the production of bioavailable folates within the seed for human consumption.

Address: UMR Qualisud, CIRAD, Univ Montpellier, TA B-95/16, 73 rue J-F. Breton, F- 34398 Montpelliercedex 5, France.; Technical University of Munich, Alte Akademie 10, 85354 Freising, Germany; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Coopers Plains, QLD 4108, Australia.; Technical University of Munich, Alte Akademie 10, 85354 Freising, Germany.; UMR QualiSud Food Process Engineering Research Unit, Montpellier SupAgro, Univ Montpellier,1101 av. Agropolis, B.P. 5098, F-34093 Montpellier cedex 5, France.; UMR Qualisud, CIRAD, Univ Montpellier, TA B-95/16, 73 rue J-F. Breton, F- 34398 Montpelliercedex 5, France. Electronic address: [email protected].

DOI: 10.1016/j.foodchem.2020.127960

Link outs

Free resources

Medical:

https://medlineplus.gov/folicacid.html

Other Literature Sources:

https://scite.ai/reports/32916403

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0308...

MeSH Terms: Folic Acid, Germination, Kinetics, Models, Biological, Seeds, Temperature, Tetrahydrofolates, Vigna