Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein.

Chao Wu, Srinivasaraghavan Kannan, Chandra S Verma, Kunchithapadam Swaminathan, Sek-Man Wong

Journal: Virology 2021;551():64-74

PMID: 33038689

Free Full Text on Elsevier Science View on PubMed

Abstract

Previous study has shown that Hibiscus sulfite oxidase (SO) interacts with Hibiscus chlorotic ringspot virus (HCRSV) coat protein (CP) and triggers sulfur enhanced defense (SED). In this study, we show the interaction of Arabidopsis SO (AtSO) and Turnip crinkle virus (TCV) CP in Arabidopsis thaliana plants. We identified the binding sites of TCV CP (W274) and AtSO (D223) using bioinformatics and confirmed it experimentally. Mutation of binding site W274 to A274 in TCV CP resulted in failure of TCV infection. TCV accumulation in SO over-expression (SO_OE) plants was lower than that in wild-type (WT) and SO knock-out (SO_KO) plants at 7 dpi but reached a level similar to that of WT and SO_KO plants at 10 dpi. AtSO competed with Argonaute 1 (AGO1) for TCV CP binding in vitro. AtSO may serve as an anti-viral factor through sequestering TCV CP for binding with AGO1 and confers virus resistance.

Address: Department of Biological Sciences, National University of Singapore, 16 Science Drive 4, 117558, Singapore.; Bioinformatics Institute (A*STAR), 30 Biopolis St, 07-01 Matrix, 138671, Singapore.; Department of Biological Sciences, National University of Singapore, 16 Science Drive 4, 117558, Singapore; Bioinformatics Institute (A*STAR), 30 Biopolis St, 07-01 Matrix, 138671, Singapore; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Dr, 637551, Singapore.; Department of Biological Sciences, National University of Singapore, 16 Science Drive 4, 117558, Singapore. Electronic address: [email protected].; Department of Biological Sciences, National University of Singapore, 16 Science Drive 4, 117558, Singapore; Temasek Life Sciences Laboratory, 1 Research Link Road, 117604, Singapore; National University of Singapore Suzhou Research Institute, Suzhou, 215123, PR China. Electronic address: [email protected].

DOI: 10.1016/j.virol.2020.08.013

Link outs

Free resources

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0042...

Miscellaneous:

https://assays.cancer.gov/CPTAC-660

Research Materials:

Subscription / membership required

Full Text Sources:

https://www.clinicalkey.com/content/playBy/pii?v=S...

Molecular Biology Databases:

https://www.arabidopsis.org/publication?key=501791...

MeSH Terms: Arabidopsis, Arabidopsis Proteins, Argonaute Proteins, Capsid Proteins, Carmovirus, Host Microbial Interactions, Models, Molecular, Plant Diseases, Protein Binding, Protein Domains, Sulfite Oxidase