SARS-CoV-2 envelope protein topology in eukaryotic membranes.

Gerard Duart, Mª Jesús García-Murria, Brayan Grau, José M Acosta-Cáceres, Luis Martínez-Gil, Ismael Mingarro

Journal: Open biology 2020;10(9):200209

PMID: 32898469

Abstract

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt/Ct). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and host components and contribute to establish the basis to tackle the pathogenesis of SARS-CoV-2.

Address: Departament de Bioquímica i Biologia Molecular, Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BioTecMed), Universitat de València E-46100 Burjassot, Spain.

DOI: 10.1098/rsob.200209

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MeSH Terms: Amino Acid Sequence, Betacoronavirus, COVID-19, Cell Membrane, Coronavirus Envelope Proteins, Coronavirus Infections, Eukaryota, Humans, Microsomes, Mutation, Pandemics, Phylogeny, Pneumonia, Viral, Recombinant Proteins, SARS-CoV-2, Sequence Alignment, Viral Envelope Proteins