Proteomic analysis of the inhibitory effect of the butanolic fraction of on .

, a plant native to northwestern Mexico, has an inhibitory effect against phytopathogenic fungi. Previous studies have shown that the butanolic extract of causes retardation and atrophy in mycelial growth of . However, the action mechanism of this extract is unknown. We used a proteomics approach to understand the inhibitory effect of butanolic extract, based on differential protein accumulation in . Proteins were extracted from cultured in Czapek broth with and without 202.12 μg/mL (IC) of butanolic extract of . Thirty-eight protein spots showing statistically significant changes (ANOVA, < 0.01) and at least a 2-fold change in abundance between experimental conditions were analyzed by mass spectrometry. Identified proteins were grouped into different biological processes according to Gene Ontology, among them were amino acid metabolism, protein folding and stabilization, protein degradation, protein transport, carbohydrate metabolism, oxidative stress response, and miscellaneous. This work is the first report of changes in the proteomic profile of exposed to the extract. This information provides new insights into the inhibitory mechanism of the extract and represents a starting point for dissection of the fungal response against the extract components.