Pretreatment of Root Canal with Photodynamic Therapy Facilitates Adhesion, Viability and Differentiation of Stem Cells of the Apical Papilla.

Yijun Li, Huan Ge, Lixuan Wu, Lishan Lei, Yanhuang Wang, Shan Jiang, Zhiyu Cai, Xiaojing Huang

Journal: Photochemistry and photobiology 2021;96(4):890-896

PMID: 32105341

Abstract

This study was to test the hypothesis that root canal pretreated with photodynamic therapy (PDT) would promote stem cells from the apical papilla (SCAP) adhesion, proliferation and differentiation without affecting smear layer removal and microhardness of root canal. Standardized root canals were randomized into four groups (n = 30/group): (1) sodium hypochlorite(NaOCl) group, (2) NaOCl + ethylene diaminetetraacetic acid (EDTA) group, (3) NaOCl + PDT group, (4) NaOCl + EDTA + PDT group. After treatments, smear layer removal and microhardness of root canal were evaluated. SCAP with hydroxyapatite-based scaffolds were seeded into root canals for 7 days. SCAP adhesion was observed by scanning electron microscope (SEM), and viable cells were calculated by CellTiter-Glo Luminescent kit. Platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) expression of SCAP were evaluated by Quantitative Reverse Transcriptase-Polymerase Chain Reaction. There was no significant difference in the smear layer removal and microhardness of root dentin between the groups with and without PDT treatment (P > 0.05). SCAP with elongated cytoplasmic processes and cell-cell contact were observed on the dentin surfaces treated with PDT. Elevated cell viability, PDGF and VEGF expression were found in root canal treated with PDT (P < 0.05). Under the experimental conditions, PDT could provide positive microenvironment for SCAP growth.

Address: Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.; Department of Stomatology, Rui Jin Hospital North, Shanghai JiaoTong University School of Medicine, Shanghai, China.; Department of Stomatology and Affiliated, Stomatological Hospital of Xiamen Medical College, Fujian, China.; Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.; Department of Stomatology, Fujian Medical University Union Hospital, Fuzhou, China.

DOI: 10.1111/php.13240

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https://doi.org/10.1111/php.13240

MeSH Terms: Cell Adhesion, Cell Differentiation, Cell Survival, Dental Pulp Cavity, Humans, Photochemotherapy, Platelet-Derived Growth Factor, Vascular Endothelial Growth Factor A