Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome.

Serena Lacerenza, Federica Ciregia, Laura Giusti, Alessandra Bonotti, Viviana Greco, Gino Giannaccini, Vanessa D'Antongiovanni, Poupak Fallahi, Luisa Pieroni, Alfonso Cristaudo, Antonio Lucacchini, Maria Rosa Mazzoni, Rudy Foddis

Journal: Cancer genomics & proteomics 2020;17(3):225-236

PMID: 32345664

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Abstract

BACKGROUND

Malignant pleural mesothelioma (MPM) a rare neoplasm linked to asbestos exposure is characterized by a poor prognosis. Soluble mesothelin is currently considered the most specific diagnostic biomarker. The aim of the study was to identify novel biomarkers by proteomic analysis of two MPM cell lines secretome.

MATERIALS AND METHODS

The protein patterns of MPM cells secretome were examined and compared to a non-malignant mesothelial cell line using two-dimensional gel electrophoresis coupled to mass spectrometry. Serum levels of candidate biomarkers were determined in MPM patients and control subjects.

RESULTS

Two up-regulated proteins involved in cancer biology, prosaposin and quiescin Q6 sulfhydryl oxidase 1, were considered candidate biomarkers. Serum levels of both proteins were significantly higher in MPM patients than control subjects. Combining the data of each receiver-operating characteristic analysis predicted a good diagnostic accuracy.

CONCLUSION

A panel of the putative biomarkers represents a promising tool for MPM diagnosis.

Address: Department of Pharmacy, University of Pisa, Pisa, Italy.; Department of Pharmacy, University of Pisa, Pisa, Italy.; Department of Rheumatology, GIGA Research, Centre Hospitalier Universitaire (CHU) de Liège, Liège, Belgium.; School of Pharmacy, University of Camerino, Camerino, Italy.; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.; Department of Translational Research and New Medical and Surgical Technologies, Occupational Medicine Unit, University-Hospital of Pisa, Pisa, Italy.; Institute of Biochemistry and Clinical Chemistry, Università Cattolica del Sacro Cuore, Rome, Italy.; Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.; Proteomics and Metabonomics Unit, IRCCS-Fondazione Santa Lucia, Rome, Italy.; Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.; Department of Pharmacy, University of Pisa, Pisa, Italy [email protected].

DOI: 10.21873/cgp.20183

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MeSH Terms: Aged, Biomarkers, Tumor, Case-Control Studies, Cell Line, Tumor, Female, GPI-Linked Proteins, Humans, Lung Neoplasms, Male, Mesothelin, Mesothelioma, Mesothelioma, Malignant, Middle Aged, Oxidoreductases Acting on Sulfur Group Donors, Pleural Neoplasms, Proteome, ROC Curve, Saposins, Secretory Pathway