Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2).

Bishajit Sarkar, Md Asad Ullah, Fatema Tuz Johora, Masuma Afrin Taniya, Yusha Araf

Journal: Immunobiology 2020;225(3):151955

PMID: 32517882

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Abstract

SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global issue which has raised the concern of scientific community to design and discover a counter-measure against this deadly virus. So far, the pandemic has caused the death of hundreds of thousands of people upon infection and spreading. To date, no effective vaccine is available which can combat the infection caused by this virus. Therefore, this study was conducted to design possible epitope-based subunit vaccines against the SARS-CoV-2 virus using the approaches of reverse vaccinology and immunoinformatics. Upon continual computational experimentation, three possible vaccine constructs were designed and one vaccine construct was selected as the best vaccine based on molecular docking study which is supposed to effectively act against the SARS-CoV-2. Thereafter, the molecular dynamics simulation and in silico codon adaptation experiments were carried out in order to check biological stability and find effective mass production strategy of the selected vaccine. This study should contribute to uphold the present efforts of the researches to secure a definitive preventative measure against this lethal disease.

Address: Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh. Electronic address: [email protected].; Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.; Department of Microbiology, School of Life Sciences, Independent University, Dhaka, Bangladesh.; Department of Genetic Engineering and Biotechnology, School of Life Sciences, Shahjalal University of Science and Technology, Sylhet, Bangladesh.

DOI: 10.1016/j.imbio.2020.151955

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MeSH Terms: Amino Acid Sequence, Betacoronavirus, COVID-19, COVID-19 Vaccines, Computational Biology, Coronavirus Infections, Disease Progression, Epitopes, Epitopes, B-Lymphocyte, Epitopes, T-Lymphocyte, HLA Antigens, Host-Pathogen Interactions, Humans, Immunogenicity, Vaccine, Molecular Docking Simulation, Pandemics, Plasmids, Pneumonia, Viral, Protein Conformation, Reverse Genetics, SARS-CoV-2, Sequence Alignment, Vaccines, Subunit, Viral Proteins, Viral Vaccines