The relevance of ice crystal formation for the cryopreservation of tissues and organs.

David E Pegg

Journal: Cryobiology 2021;93():3-11

PMID: 32329718

Full Text on Elsevier Science View on PubMed

Abstract

This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differently from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs.

Address: Department of Biology, University of York, YO10 5YW, UK. Electronic address: [email protected].

DOI: 10.1016/j.cryobiol.2020.01.005

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MeSH Terms: Cryopreservation, Freezing, Humans, Ice, Kidney, Microcirculation, Organ Preservation