Effects of Selenium on Differentiation and Antioxidant Activity of Sclerotium of Q1 Strain.

Selenium is an essential trace element, which has certain antioxidant properties. NaSeO is toxic, and its use is limited. SeMet, as an organic selenium, is less toxic than NaSeO. In this experiment, different concentrations of NaSeO and SeMet were added to MEA and PDA media to observe the effect of selenium on the sclerotium differentiation of Q1 strain, and the contents of carotenoids, ascorbic acid, and total phenol and their reducing power, DPPH free radical scavenging ability, ferrous ion chelating ability, and superoxide anion scavenging ability were determined. Meanwhile, the orthogonal design was used to optimize the selenium enrichment culture conditions of Q1. The results showed that the addition of selenium in the PDA medium was not conducive to the differentiation of Q1 strain. The addition of inorganic and organic selenium in the MEA medium at different concentrations resulted in the accumulation of carotenoids, ascorbic acid, phenols, and selenium in the sclerotia of Q1 strain, and the contents of carotenoids, ascorbic acids, and selenium in the sclerotia of Q1 strain were increased to different degrees, but it cannot increase the content of total phenol. In addition, when the concentration of NaSeO and SeMet in the medium was 10 g/mL, the reducing power of the extract was improved. The experimental results can provide a new research idea for the utilization and development of Penicillium sclerotium and selenium.

Copyright © 2020 Wenjing Zhao et al.